- 2 Ubiquitin-like Protein Specific Protease UfSP2 and Process for Preparing the Same

摘要

A novel protease is provided to show the specific protease activity on Ufm1, which acts on an important mechanism in a cell, thereby being widely utilized for investigating the important mechanism in the cell where the Ufm1 involves. A Ufm1-specific protease 2(UfSP2) includes an amino acid sequence of SEQ ID : NO. 1. A method for preparing the UfSP2 comprises the steps of: (a) culturing a transformant which is prepared by transforming E. coli BL21 using an expression plasmid of pMAL-c2X-UfSP2; and (b) obtaining the UfSP2 from the cultured fungus bodies.