Primer and probe for detection of Bacillus anthracis and method for detecting Bacillus anthracis using thereof

摘要

A method for detecting Bacillus anthracis is provided to detect the Bacillus anthracis specifically and distinguish B. cereus, which is very similar to the Bacillus anthracis taxonomically and molecular biologically, from the Bacillus anthracis. A probe for detecting Bacillus anthracis is at least one selected from the group consisting of: (a) a sequence coding an amino acid sequence of SEQ ID : NO. 2; (b) a sequence complimentary to the sequence of the (a); and (c) a consecutive sequence more than 20bp which is derived from the sequence of the (a) or (b) and includes a sequence selected from the group consisting of sequences of SEQ ID : NOs. 3 to 44. A primer for detecting Bacillus anthracis includes a sequence selected from the group consisting of sequences of SEQ ID : NOs. 3 to 44. To detect Bacillus anthracis, at least one of the probe and the primer is used through PCR(Polymerase Chain Reaction), DNA sequencing, hybridization by microarray, PCR-RFLP(restriction fragment length polymorphism), RAPD(randomly amplified polymorphic DNA), DAF(DNA amplification fingerprinting), AP-PCR(arbitrarily primed PCR), STS(sequence tagged site), EST(expressed sequence tag), SCAR(sequence characterized amplified regions), ISSR(inter-simple sequence repeat amplication), AFLP(amplified fragment length polymorphism), CAPS(cleaved amplified polymorphic sequence), PCR-SSCP(single-strand conformation polymorphism), Northern hybridization or Southern hybridization. Further, the probe is accumulated on a substrate.