MULTIPOTENT STEM CELL OR CANCER STEM CELL SIMULTANEOUSLY EXPRESSING CRIPTO-1 AND OCT4 DERIVED FROM HUMAN ADIPOSE TISSUE OR CANCER AND METHOD FOR IDENTIFYING THE SAME

摘要

A method for identifying an adult stem cell is provided to express Oct4, which is a marker to a multipotent stem cell, as well as Cripto-1, which is a marker for indifferentiation state, in the adult stem cell, thereby being useful for identifying more closely genuine adult stem cell. A method for identifying and isolating adult stem cells or tumor stem cells derived from human adipose tissue comprises the steps of: (a) immunostaining a group of adult stem cell or tumor stem cell candidates derived from the adipose tissue using an antibody binding to Cripto-1 and Oct4; (b) identifying the stem cell candidates group showing positive characteristic both on the Cripto-1 and Oct4 as the adult stem cells or tumor stem cells derived from the adipose tissue using a flow cytometer; and (c) isolating the identified stem cells.