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Qualitative and quantitative detection for GM rice products

机译:转基因大米产品的定性和定量检测

摘要

The present invention has been spent and, more particularly rice inherent transgenes and foreign by the test method a nucleic acid polymerase chain reaction (PCR) method from the rice chromosome DNA of the transgenic agricultural products on the qualitative and quantitative analysis of transgenic rice plants different by using a primer set to amplify a region containing all or part of the DNA region of the endogenous gene and the object region gene in whole or in part, with another expression control in a manner that checks the transgenic rice plants by detecting the gene specifically to provides a method, it characterized in that it can be detected to determine the recombinant gene of the strains. Transgenic rice black PCR primers, probes, standard plasmid of the present invention may be used for the screening of not only qualitative and quantitative analysis of the transgenic rice plants that are able to commercialization, and distribution to the GMO test kit homozygous lines (homozygote line) Rice can. ; Transgenic rice, PCR, real time PCR, specific primers, probes, GMO black, homozygous (homozygote), endogenous gene, transgene
机译:本发明已经用掉了,更具体地说是水稻固有的转基因和外来的测试方法,通过核酸聚合酶链反应(PCR)法从转基因农产品的水稻染色体DNA上对不同转基因水稻进行定性和定量分析通过使用引物组来扩增全部或部分包含内源基因和对象区域基因的全部或部分DNA区域的区域,并通过另一种表达控制方式,通过特异性检测基因来检查转基因水稻植株提供一种方法,其特征在于可以对其进行检测以确定菌株的重组基因。本发明的转基因水稻黑PCR引物,探针,标准质粒不仅可用于筛选能够商业化的转基因水稻植物的定性和定量分析,而且还可用于纯合子系(纯合系)饭可以。 ;转基因水稻,PCR,实时PCR,特异性引物,探针,GMO黑,纯合子(纯合子),内源基因,转基因

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