Real-time dual color assay for detecting protein-protein interaction and an apparatus therefor

摘要

Antigen A present invention with a fluorescent label of the wavelength X; The first antibody is coupled to the anti-antigen A -A; And the second anti-antibody protein consisting of -B with a fluorescent label Y having a wavelength coupled to the first antibody anti--A - in real-time bi-color analysis method for measuring a protein interaction, the double-color analysis with the sample measured by an enzyme immune response to the protein interaction, and the result of the dual wavelength X and Y - to provide a real-time bi-color analysis method and apparatus characterized by: reading from the color of the wavelength. In addition, the present invention is a double-molecule to provide a real-time method that can be bi-color analysis using a fluorescence detection system in real time a fluorescent image of the individual protein molecule is monitored at the same time without time delay of the other labeled with a fluorescent dye-single wavelength. ; Protein-protein interactions, dual-color, analysis, enzyme, fluorescence, kit