REAL-TIME DUAL COLOR ASSAY FOR DETECTING PROTEIN-PROTEIN INTERACTION AND AN APPARATUS THEREFOR

摘要

A dual color assay method is provided to detect real-time dynamics of temperature-dependent protein-protein interaction in solution in the single-molecule level by using a dual-wavelength laser-induced fluorescence, so that real-time fluorescence images of individual protein molecule labeled with different fluorescence dyes are monitored simultaneously without delay of time. A dual color assay method for detecting protein-protein interaction comprises the steps of: measuring the interaction between an analyte antigen protein and an antibody protein by using enzyme-linked immunosorbent assay; and interpreting the measured results by using the double-wavelength color of wavelength X and Y selected from 440 nm, 488 nm, 545 nm, 580 nm and 635 nm, wherein the enzyme-linked immunosorbent assay contains an analyte protein antigen A having a fluorescence label of wavelength X, a first antibody protein anti-A binding to the analyte protein antigen A, and a second antibody protein anti-B having a fluorescence label of wavelength Y, binding to the first antibody protein anti-A; and the analysis temperature is 12-32 deg. C.