METHOD FOR PRODUCING L-amino acids using bacteria FAMILY ENTEROBACTERIACEAE, IN WHICH THE EXPRESSION weakened gene cluster YEHABCDE

摘要

1. u0431u0430u043au0442u0435u0440u0438u00a0 - a producer of l - amino acids, u043fu0440u0438u043du0430u0434u043bu0435u0436u0430u0449u0430u00a0 to the family Enterobacteriaceae, u043cu043eu0434u0438u0444u0438u0446u0438u0440u043eu0432u0430u043du043du0430u00a0 in such a way that u044du043au0441u043fu0440u0435u0441u0441u0438u00a0 cluster genes in bacteria of the yehABCDE u0441u043bu0430u0431u043bu0435u043du0430.;2. u0431u0430u043au0442u0435u0440u0438u00a0 on 1, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0, u044du043au0441u043fu0440u0435u0441u0441u0438u00a0 yehABCDE weakened through the cluster of genes in the cluster of genes yehABCDE.;3. u0431u0430u043au0442u0435u0440u0438u00a0 on 1, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0, u0443u043au0430u0437u0430u043du043du0430u00a0 u0431u0430u043au0442u0435u0440u0438u00a0 belongs to the genus escherichia.;4. u0431u0430u043au0442u0435u0440u0438u00a0 on 1, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0, u0443u043au0430u0437u0430u043du043du0430u00a0 u0431u0430u043au0442u0435u0440u0438u00a0 belongs to the family Pantoea.;5. u0431u0430u043au0442u0435u0440u0438u00a0 - a producer of l - amino acids on any of the u043fu043f.1 - 4, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0, u0443u043au0430u0437u0430u043du043du0430u00a0 l - amino acid selected from the group of u0441u043eu0441u0442u043eu00a0u0449u0435u0439 (l - amino acids and n u0435u0430u0440u043eu043cu0430u0442u0438u0447u0435u0441u043au043eu0439 l - amino acids.;6. u0431u0430u043au0442u0435u0440u0438u00a0 - a producer l - amino acids on clause 5, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0, u0443u043au0430u0437u0430u043du043du0430u00a0 u0430u0440u043eu043cu0430u0442u0438u0447u0435u0441u043au0430u00a0 l - amino acid selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of l - u0444u0435u043du0438u043bu0430u043bu0430u043du0438u043du0430, l - u0442u0438u0440u043eu0437u0438u043du0430 and l - t u0440u0438u043fu0442u043eu0444u0430u043du0430.;7. u0431u0430u043au0442u0435u0440u0438u00a0 - a producer l - amino acids on clause 5, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0, u0443u043au0430u0437u0430u043du043du0430u00a0 u043du0435u0430u0440u043eu043cu0430u0442u0438u0447u0435u0441u043au0430u00a0 l - amino acid selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of l - u0442u0440u0435u043eu043du0438u043du0430, l - lysine, l - u0446u0438u0441u0442u0435u0438 a, l - methionine, l - u043bu0435u0439u0446u0438u043du0430, l - u0438u0437u043eu043bu0435u0439u0446u0438u043du0430, l - valin, l - u0433u0438u0441u0442u0438u0434u0438u043du0430, l - u0433u043bu0438u0446u0438u043du0430, l - serina, l - u0430u043bu0430u043du0438u043du0430, l - u0430u0441u043fu0430u0440u0430u0433u0438u043du0430, l - u0430u0441u043fu0430u0440u0442u0430u0442u0430, l - u0433u043bu0443u0442u0430u043cu0438u043du0430, l - u0433u043bu0443u0442u0430u043cu0438u043du043eu0432u043eu0439 acid, l - and l - u043fu0440u043eu043bu0438u043du0430 arges u0438u043du0438u043du0430.;8. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 l - amino acids, including;the cultivation of the bacteria in any of paragraphs. from 1 to 7 in the nutritional environment that causes the production and accumulation of the l - amino acids in u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du043eu0439 liquids; and;the allocation of the l - amino acids from u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du043eu0439 fluid.;9. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 point 9, so that the u0443u043au0430u0437u0430u043du043du0430u00a0 l - amino acid selected from the group of u0441u043eu0441u0442u043eu00a0u0449u0435u0439 (l - amino acids and u043du0435u0430u0440u043eu043cu0430u0442u0438u0447u0435u0441u043au043eu0439 l - amino acids.;10. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 p.9, so that u0443u043au0430u0437u0430u043du043du0430u00a0 u0430u0440u043eu043cu0430u0442u0438u0447u0435u0441u043au0430u00a0 l - amino acid selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of l - u0444u0435u043du0438u043bu0430u043bu0430u043du0438u043du0430, l - u0442u0438u0440u043eu0437u0438u043du0430 and l - tryptophan.;11. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 p.9, so that u0443u043au0430u0437u0430u043du043du0430u00a0 u043du0435u0430u0440u043eu043cu0430u0442u0438u0447u0435u0441u043au0430u00a0 l - amino acid selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of l - u0442u0440u0435u043eu043du0438u043du0430, l - lysine, l - u0446u0438u0441u0442u0435u0438u043du0430, l - methionine, l - u043bu0435u0439u0446u0438u043du0430, l - u0437u043eu043bu0435u0439u0446u0438u043du0430, l - valin, l - u0433u0438u0441u0442u0438u0434u0438u043du0430, l - u0433u043bu0438u0446u0438u043du0430, l - serina, l - u0430u043bu0430u043du0438u043du0430, l - u0430u0441u043fu0430u0440u0430u0433u0438u043du0430, l - u0430u0441u043fu0430u0440u0442u0430u0442u0430, l - u0433u043bu0443u0442u0430u043cu0438u043du0430, l - u0433u043bu0443u0442u0430u043cu0438u043du043eu0432u043eu0439 acid, l - u043fu0440u043eu043bu0438u043du0430 and l - u0430u0440u0433u0438u043du0438u043du0430.