METHOD FOR PRODUCING RNase Bacillus intermedius (binase) using recombinant strains of Escherichia coli SURE pML 163

摘要

1. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 u0440u043du043au0430u0437u044b B.intermedius (u0431u0438u043du0430u0437u044b) using a strain of e. coli with, including the transformation strain of e. coli sure u043fu043bu0430u0437u043cu0438u0434u043du043eu0439 dna pML163 containing structure crew u0431u0438u043du0430u0437u044b gene under tac - u043fu0440u043eu043cu043eu0442u043eu0440u043eu043c and phoA - signal sequence of e. coli.;2. the cultivation of the strain - u043fu0440u043eu0434u0443u0446u0435u043du0442u0430 rich nutritive medium containing sources of carbon, nitrogen, phosphorus, and biologically active substances and the distilled the water u043eu0442u043bu0438u0447u0430u044eu0449u0435u0435u0441u00a0, as a source of carbon and nitrogen u0438u0441u043fu043eu043bu044cu0437u0443u0435u0442u0441u00a0 u0431u0430u043au0442u0435u0440u0438u043eu043bu043eu0433u0438u0447u0435u0441u043au0438u0439 u043fu0435u043fu0442u043eu043d not depleted phosphorus and as a source of neo u0440u0433u0430u043du0438u0447u0435u0441u043au043eu0433u043e phosphorus u0438u0441u043fu043eu043bu044cu0437u0443u0435u0442u0441u00a0 Na2HPO4.