POLYNUCLEOTIDE, CODING CHROMO- OR FLUORESCENT MUTANT POLYPEPTIDE, EXPRESSION VECTOR, CELL, METHOD OF OBTAINING CHROMO- OR FLUORESCENT POLYPEPTIDE, USE OF POLYPEPTIDE AND USE OF POLYNUCLEOTIDE

摘要

FIELD: chemistry.;SUBSTANCE: polynucleotide is obtained, coding chromo- or fluorescen mutant wild type DsRed (SEQ ID N0:2), where chromo- or fluorescent mutant contains a substitute in the amino acid position 42 SEQ ID N0:2, and optionally one or more substitutes in the amino acid positions, chosen from a group, consisting of 4, 2, 5, 6, 21, 41, 44, 117, 217, 145. Using the polynucleotide in the vector, the host cells which express chromo- or fluorescent mutant DsRed are transformed. The invention allows for obtaining chromo- or fluorescent polypeptide DsRed, which matures faster than wild type DsRed.;EFFECT: increased effectiveness.;26 cl, 10 dwg, 2 tbl, 4 ex