METHOD OF CYSTIC ECHINOCOCCOSIS CAUSATIVE AGENT IDENTIFICATION IN BIOLOGICAL SAMPLE

摘要

FIELD: biology.;SUBSTANCE: invention concerns molecular biology and can be applied in epidemiology. It claims a method of cystic echinococcosis agent identification involving extraction of DNA from ehinococcus larvocyst cells and polymerase chain reaction (PCR) using primers specific to mitochondrial gene CO1 of Echinococcus granulosus. Oligonucleotides with sequences 5' TGTGTTGATTTTGCCTGG 3' and 5' GCCACCACAAACCAAGTATC 3' are applied as direct and reverse primers respectively, as they are more universal in relation to various isolates of disease causative agents.;EFFECT: increased analysis reliability for population research in different regions.;2 ex