Method for isolating plasmid DNA from bacterial lysate, involves resuspending bacterial cells in standard buffer, and bacterial cells are lysed with standard buffer e.g. sodium dodecyl sulphate or sodium hydroxide

摘要

Plasmid DNA isolation involves resuspending bacterial cells in a standard buffer. The bacterial cells are lysed with the standard buffer e.g. sodium dodecyl sulphate (SDS) or sodium hydroxide or by hydrolase e.g. lysozyme which affects the cell wall of bacteria. The lysate is mixed with a binding buffer on the base of a salt-alcohol-mixture with acidic pH-value, so that pH-value, after mixing with lysis buffer, amounts to less than 8. The linked plasmid DNA is washed with a buffer, preferably alcohol containing buffer. The plasmid is extracted by a low salt buffer or water. An independent claim is also included for a kit for isolating plasmid DNA which comprises a standard buffer for resuspending bacterial cell, and a binding buffer on the base of a salt-alcohol-mixture with acidic pH-value.