A DNA fragment containing a toluene monooxygenase gene, a recombinant plasmid, a transformed microorganism, a method for degrading chlorinated aliphatic hydrocarbons and aromatic compounds, and methods of environmental containment

摘要

A recombinant DNA is constructed by using a toluene monooxygenase gene isolated from Burkholderia cepacia strain KK01 and employed to provide the transformant which can express toluene monooxygenase useful for cleaning of aqueous media such as drain and waste water containing chlorinated aliphatic hydrocarbon compounds or aromatic compounds, for remediation of soil polluted with such compounds, and cleaning of air (gas phase) polluted with volatile organic chlorine compounds.