CELL FOR GENE THERAPY OF LCAT DEFICIENCY, AND REPLICATION-DEFICIENT RETROVIRUS VECTOR AND PLASMID FOR USE IN PRODUCTION OF THE CELL

摘要

PPROBLEM TO BE SOLVED: To provide a replication-deficient retrovirus vector carrying an LCAT-encoding nucleotide, which enables the LCAT-encoding nucleotide as an exogenous gene to be expressed in a target cell with efficiency, and which can be used for the production of cells for gene therapy to be administered to a patient, and a plasmid which can be used for the production of the retrovirus vector. PSOLUTION: Provided is the plasmid for the production of the replication-deficient retrovirus vector, having an LCAT-encoding nucleotide sequence which is inserted into between the 5'-LTR sequence and the 3'-LTR sequence originating from the retrovirus, as an exogenous gene, and a Kozak's consensus sequence and a packaging signal sequence for the retrovirus both are linked to the 5'-end of the LCAT-encoding nucleotide sequence. PCOPYRIGHT: (C)2009,JPO&INPIT