CHIMERIC MOLECULE CONTAINING MODULE ABLE TO TARGET SPECIFIC CELL AND MODULE REGULATING APOPTOGENIC FUNCTION OF PERMEABILITY TRANSITION PORE COMPLEX (PTPC)

摘要

PROBLEM TO BE SOLVED: To provide an apoptosis regulating molecule for therapeutic use acting on a permeability transition pore complex (PTPC).;SOLUTION: Provided are a chimeric polypeptide has the formula: pTox-pTarg (wherein, pTox is a viral apoptotic peptide such as the Vpr peptide of HIV-1, or a fragment of the Vpr peptide of HIV-1 containing the amino acid motif H(F/S)RIG that interacts with mitochondrial inner membrane, adenine nucleotide translocation (ANT) protein of a cell, and pTarg is an antibody or an antibody fragment that binds to the outer membrane of the cell), a vector encoding the chimeric polypeptide, and a recombinant host cell comprising the vector. Binding of the chimeric polypeptide to the cell is followed by apoptosis of the cell. The chimeric peptide is useful for targeting pTox to cells, such as cancer cells.;COPYRIGHT: (C)2009,JPO&INPIT