Being the sample injection manner which injects

摘要

PROBLEM TO BE SOLVED: To provide a sample injection method which enables the reduction in size of a micro device to be used, accurate analysis of the micro device and inexpensive implementation thereof, as well as a small micro device which enables accurate analysis.;SOLUTION: A buffer is introduced in a first opening part 16, and the first opening part 16 is pressurized to fill the buffer in an injection path 24. While inhibiting the buffer in the injection path 24 and a second opening part 18 from being substantially moved in an introduction flow path 28 or a quantitative path 26, a sample is introduced in a third opening part 20. The buffer is introduced in the first and second opening parts 16, 18, and voltage is applied between the buffer in the first opening part 16 and the buffer in the second opening part 18. Further, the third opening part 20 is pressurized to fill a sample in the introduction flow path 28 and quantitative path 26 while inhibiting the buffer in the injection path 24 from being substantially moved. By an electroendosmosis phenomenon, a quantitative amount of the sample in the quantitative path 26 is separated from the sample in the introduction flow path 28 to move the quantitative amount of the sample in a main flow path 22 from the second opening part 18 in a direction toward the first opening part 16 for the injection of a quantitative amount of the sample.;COPYRIGHT: (C)2005,JPO&NCIPI