Microwave-assisted deglycosylation of proteins for molecular weight determination by mass spectrometry

摘要

Is to provide a microwave-assisted enzymatic deglycosylation method of protein. The speed to achieve deglycosylation without denaturing proteins, rapid and accurate mass spectrometric molecular weight determination becomes easy. Was treated with the enzyme glycosylated proteins under microwave conditions, whereby the present invention, which comprises a step of forming a deglycosylated protein, deglycosylation methods of protein. Can be applied to a desalting media, eluted, and analyzed by mass spectrometry, the deglycosylated protein.