首页> 外国专利> Method for producing specific antiserum against universal tumor antigen and method for diagnosing malignant tumor using said antiserum

Method for producing specific antiserum against universal tumor antigen and method for diagnosing malignant tumor using said antiserum

机译:产生针对通用肿瘤抗原的特异性抗血清的方法和使用所述抗血清诊断恶性肿瘤的方法

摘要

The present invention pertains to the field of medicine and may be used for producing a specific antiserum as well as for carrying out immunological diagnoses of malignant tumours. This method for producing an antiserum involves sampling an embryo at the foetal stage from animals of a same genetic type so as to obtain a cell suspension. After immunisation, this method involves sampling spleen cells from the animal, separating lymphocytes and immunising the animal of the same genetic line using the lymphocyte suspension. An antiserum is then obtained and cells originating from healthy organs of the same animals are added to said antiserum. The mixture is finally decanted and the liquid located above the sediments is filtered. In order to carry out a diagnosis, the filtrate is added to the subject's blood and the results are obtained by immuno-fluorescence, by blood tests or using other methods of immunological diagnosis. It it thus possible to diagnose a tumour when the reliable values obtained differ from reference values.
机译:本发明涉及医学领域,并且可以用于产生特定的抗血清以及用于进行恶性肿瘤的免疫学诊断。这种产生抗血清的方法涉及从相同遗传类型的动物的胎儿期取样胚胎,以获得细胞悬液。免疫后,该方法涉及从动物中采集脾细胞,分离淋巴细胞,并使用淋巴细胞悬液免疫同一基因系的动物。然后获得抗血清,并将源自相同动物的健康器官的细胞添加至所述抗血清。最后将混合物倒出并将位于沉积物上方的液体过滤。为了进行诊断,将滤液添加到受试者的血液中,并通过免疫荧光,血液测试或使用其他免疫学诊断方法获得结果。因此,当获得的可靠值与参考值不同时,可以诊断出肿瘤。

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