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Method for producing specific antiserum against universal tumor antigen and method for diagnosing malignant tumor using said antiserum
Method for producing specific antiserum against universal tumor antigen and method for diagnosing malignant tumor using said antiserum
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机译:产生针对通用肿瘤抗原的特异性抗血清的方法和使用所述抗血清诊断恶性肿瘤的方法
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摘要
The present invention pertains to the field of medicine and may be used for producing a specific antiserum as well as for carrying out immunological diagnoses of malignant tumours. This method for producing an antiserum involves sampling an embryo at the foetal stage from animals of a same genetic type so as to obtain a cell suspension. After immunisation, this method involves sampling spleen cells from the animal, separating lymphocytes and immunising the animal of the same genetic line using the lymphocyte suspension. An antiserum is then obtained and cells originating from healthy organs of the same animals are added to said antiserum. The mixture is finally decanted and the liquid located above the sediments is filtered. In order to carry out a diagnosis, the filtrate is added to the subject's blood and the results are obtained by immuno-fluorescence, by blood tests or using other methods of immunological diagnosis. It it thus possible to diagnose a tumour when the reliable values obtained differ from reference values.
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