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Detection method of the 3 adrenaline receptor mutation gene and the nuclear acid probe and kit null end
Detection method of the 3 adrenaline receptor mutation gene and the nuclear acid probe and kit null end
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机译:3肾上腺素受体突变基因的检测方法及核酸探针和试剂盒无效末端
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摘要
A melting curve analysis is performed for a nucleic acid containing a mutation in a nucleotide sequence resulting in a mutation replacing tryptophan at position 64 in an amino acid sequence of the ²3-adrenergic receptor with arginine (B3AR Trp64Arg), by using a nucleic acid probe of which end is labeled with a fluorescent dye, and in which fluorescence of the fluorescent dye decreases upon hybridization, wherein the nucleic acid probe has a nucleotide sequence starting from the nucleotide number 183 in the nucleotide sequence of SEQ ID NO: 1 and having a length of 8 to 30 nucleotides, and the 5' end of the probe is labeled with the fluorescent dye, or the nucleic acid probe has a nucleotide sequence ending at the nucleotide number 196 in the nucleotide sequence of SEQ ID NO: 2 and having a length of 7 to 30 nucleotides, and the 3' end of the probe is labeled with the fluorescent dye, and measuring fluorescence of the fluorescent dye, and the mutation is detected on the basis of the result of the melting curve analysis.
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机译:通过使用核酸探针,对核苷酸序列中包含突变的核酸进行了熔解曲线分析,该核酸导致突变将3-3-肾上腺素能受体的氨基酸序列中第64位的色氨酸替换为精氨酸(B3AR Trp64Arg)其末端被荧光染料标记,并且其中荧光染料的荧光在杂交时降低,其中所述核酸探针具有从SEQ ID NO:1的核苷酸序列中的核苷酸编号183开始的核苷酸序列。长度为8至30个核苷酸,并且探针的5'端用荧光染料标记,或者核酸探针的核苷酸序列以SEQ ID NO:2的核苷酸序列中的核苷酸编号196结尾。长度为7至30个核苷酸,并在探针的3'端标记荧光染料,并测量荧光染料的荧光,并根据res检测突变熔解曲线分析的全部。
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