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Extract for cell-free protein synthesis, cell-free protein synthesis method using the same, and method for preparing the extract

机译:用于无细胞蛋白质合成的提取物,使用其的无细胞蛋白质合成方法以及制备该提取物的方法

摘要

PROBLEM TO BE SOLVED: To provide an easily prepared new extract for protein synthesis by a cell-free system, and enabling the protein synthesis by the cell-free system by which a glycoprotein can also be synthesized, to be achieved, and to provide a method for preparing the extract, and a method for the protein synthesis by the cell-free system by using the extract.;SOLUTION: The extract for the protein synthesis by the cell-free system contains at least an extract derived from a silkworm tissue, and an extraneous mRNA. The method for protein synthesis by the cell-free system uses the extract. The method for preparing the extract for the protein synthesis by the cell-free system involves adding the extraneous mRNA to the extract derived from the silkworm tissue and extracted from the silkworm tissue by using a liquid for extraction.;COPYRIGHT: (C)2003,JPO
机译:要解决的问题:提供一种易于制备的新提取物,用于通过无细胞系统进行蛋白质合成,并实现通过无细胞系统进行蛋白质合成,通过该系统还可以合成糖蛋白,并提供一种提取物的制备方法,以及使用该提取物的无细胞系统的蛋白质合成方法。解决方案:无细胞系统的蛋白质合成的提取物至少含有来源于蚕组织的提取物,和无关的mRNA通过无细胞系统合成蛋白质的方法使用提取物。通过无细胞系统制备用于蛋白质合成的提取物的方法包括,将外源mRNA添加到通过使用提取液从家蚕组织提取并从家蚕组织提取的提取物中。COPYRIGHT:(C)2003,日本特许厅

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