To transcribe mRNA of 1 or more from mold DNA in the non cell protein synthesis system which includes the protein or peptide null

摘要

PROBLEM TO BE SOLVED: To provide a method for producing a protein or peptide in a cell-free protein synthesis using a cell-free extract in excellent protein synthesis efficiency.;SOLUTION: This method for producing the protein or peptide comprises carrying out a RNA polymerase reaction using a cyclic ssDNA having a sequence complementary to the code sequence of the protein or peptide as a template to synthesize one or more mRNAs in which the complementary sequences of the cyclic ssDNA are transcribed on one mRNA, and synthesizing the protein or peptide in the cell-free protein synthesis system using the cell-free extract in the presence of the mRNA as a template.;COPYRIGHT: (C)2004,JPO