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Cold shock-inducible expression and production of heterologous polypeptide
Cold shock-inducible expression and production of heterologous polypeptide
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机译:冷休克诱导表达及异源多肽的产生
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摘要
The present invention relates to host cells containing capable of producing a heterologous polypeptide under conditions and using it, to induce cold-shock response in the host cell, the vector DNA or the vector and DNA molecule or. Vector DNA and molecules, including the 5'-UTR and promoter of direct cold shock-inducible genes derived from its expression as well as the nucleotide sequence encoding a heterologous polypeptide. Furthermore, additional elements in the coding sequence of a heterologous polypeptide, AT-rich sequences that enhance translation in the cold-shock-induced conditions, is inserted between or with the 5'-UTR and cold shock inducible promoter and coding sequence I will present in any of.
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