Epi-fluorescence and reflection contrast observable microscope and how to use the microscope

摘要

PROBLEM TO BE SOLVED: To provide a microscope which enables reflection contrast lighting observation by using a unit of a epifluorescent lighting device in common and only making simple constitution alternations.;SOLUTION: An intermediate optical system is arranged between a light source which lights up a sample and an objective, and the intermediate optical system comprises a relay optical system, an aperture stop member, an exciting filter element which transmits an exciting component of the illumination light of the light source, a reflecting optical element which guides the exciting component to the sample, limits exciting light of reflected light reflected by the sample, and guides the remaining fluorescent light to an observation system, and a barrier filter element which cuts light wavelength components other than fluorescent light from the reflecting optical element and transmits the fluorescent light.;COPYRIGHT: (C)2004,JPO