C-terminal modification method of peptide, analysis and C-terminal immobilization method, or protein

摘要

How cheap, isolated easily and surely C-terminal modification method of peptide, the C-terminal peptide fragment of a protein or peptide or protein efficient and simple, high reliability and accuracy and quick and using the mass spectrometer I provides the amino acid sequencing method of protein-peptide. By peptides or proteins, the addition of a catalyst and a formylation reagent, and method for converting the aldehyde group to a carboxyl group. By reacting a nucleophilic reagent to the aldehyde group, the method of modifying the C-terminus of the protein or peptide. By reacting the support with a nucleophilic group to the aldehyde group, the method for immobilizing the protein or peptide. Methods for digesting a protein or peptide is the fixed, washing the carrier, is isolated from the carrier C-terminal peptide fragment. How to mass spectrometry C-terminal peptide fragment isolated, perform the amino acid sequencing.