Flat-field correction of the two-dimensional biochemical analysis image

摘要

Optical image of the two-dimensional solute zone in the electrophoresis gel is corrected for non-uniformity, such as caused by light dispersion under the gel or light source. Correction is performed using the reference plate, the reference plate with respect to the light source in the direction of length and width by emitting light by passing through the uniform light or be excited or absorb uniformly light I will respond uniformly. Therefore, deviation or nonuniformity in the image of the reference plate resulting from the deviation or by non-uniformity of the optical system. A similar correction is performed and a microtiter array or microarray, in the two-dimensional image analysis of the other.