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DETECTION OF FOOD SPECIFIC HUMAN IgG4 ANTIBODIES
DETECTION OF FOOD SPECIFIC HUMAN IgG4 ANTIBODIES
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机译:食物特异性人IgG4抗体的检测
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摘要
This invention particularly discloses an improved immunoassay method for the sensitive and specific detection of food specific human IgG4 antibodies. A sample diluent comprising a chaotrophic agent is used to reduce the occurrence of nonspecific antibody-dietary antigen interactions. To reduce competition between IgE and IgG4 antibodies for specific epitopes on dietary antigens a heat denaturing step is included to inactivate IgE antibodies. Finally, a signal amplification step is included in the assay to reduce the amount of sample required to perform the assay.
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