TERMINATION OF TRANSGENE EXPRESSION VIA TRANSPOSON-MEDIATED BREAK

摘要

This invention provides a genetic construct comprising: (a) a DNA cut-and-paste transposon genetic unit which comprises a transposase gene flanked on either side by inverted repeat sequences and operably under the control of a first promoter; and (b) a transgene unit which comprises an expressable transgene, placed operably under the control of a second promoter; wherein the transposon genetic unit and the transgene unit are linked with one of the inverted repeat sequence located in an intron of the transgene.;This invention also provides methods of transiently expressing a transgene in a stably transformed eukaryote.;This invention further provides methods for obtaining marker-free transgenic plants.