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COLORIMETRIC MEASUREMENT OF ENZYMATIC ACTIVITY IN CELL LYSATES
COLORIMETRIC MEASUREMENT OF ENZYMATIC ACTIVITY IN CELL LYSATES
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机译:比色法测定细胞裂解液中酶的活性
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摘要
The present invention concerns a method for the calorimetric measurement of enzyme activity comprising the following steps: preparing a cell suspension or a cell lysate containing cell components, adding a chromogenic substrate at the start of the enzyme reaction, adding a first additional substance to stop the enzyme reaction, and making a colorimetric measurement, wherein a second additional substance which reduces the light scattering and/or absorption caused by the cells or the cell components is added during or after termination of the enzyme reaction.
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