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Method for obtaining information regarding quantity of DNA after non-methylated cytosine converting treatment in analysis of DNA methylation

机译:DNA甲基化分析中非甲基化胞嘧啶转化处理后获得DNA量信息的方法

摘要

The present invention provides an easy and accurate method for obtaining information regarding a quantity of DNA included in a sample used for analyzing a methylation of a target DNA, comprising steps of: treating a biological sample containing DNA including the target DNA with a non-methylated cytosine converting agent for converting non-methylated cytosine of the DNA into another base; and obtaining information regarding a quantity of DNA included in the sample treated in the treating step by using an oligonucleotide complementary to a cytosine-free region of the target DNA.
机译:本发明提供一种用于获得关于用于分析靶DNA的甲基化的样品中包括的DNA的量的信息的简便而准确的方法,该方法包括以下步骤:用非甲基化处理包含DNA的生物样品,所述DNA包括靶DNA。胞嘧啶转化剂,用于将DNA的非甲基化胞嘧啶转化为另一个碱基。通过使用与靶DNA的无胞嘧啶区域互补的寡核苷酸,获得关于在处理步骤中处理的样品中包括的DNA量的信息。

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