首页> 外国专利> Method for in vitro phosphorylation of TRAP of Staphylococcus aureus and a method for screening the inhibitor of the TRAP phosphorylation

Method for in vitro phosphorylation of TRAP of Staphylococcus aureus and a method for screening the inhibitor of the TRAP phosphorylation

机译：金黄色葡萄球菌的TRAP的体外磷酸化方法和筛选TRAP磷酸化抑制剂的方法

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摘要

The present invention relates to a method for in vitro phosphorylation of TRAP derived from Staphylococcus aureus. In the present invention, the TRAP is first identified as a kinase that self-phosphorylates and is phosphorylated specifically in the presence of an oxidative metal ion such as iron, copper and zinc. Based upon this finding, a novel method for in vitro phosphorylation of purified TRAP is provided and also, a method for screening various chemicals and natural materials by using the above method is provided in order to select inhibitors against the TRAP phosphorylation. The inhibitors are expected to be applied for novel antibiotics of Staphylococcus aureus, because this TRAP phosphorylation is essential to infect Staphylococcus aureus. Therefore, the present invention can be widely used to develop novel drugs against Staphylococcus aureus and their resistant strains, in near future.

机译：本发明涉及一种源自金黄色葡萄球菌的TRAP的体外磷酸化的方法。在本发明中，首先将TRAP鉴定为在存在氧化性金属离子（例如铁，铜和锌）的情况下自磷酸化并特别被磷酸化的激酶。基于该发现，提供了一种用于纯化TRAP的体外磷酸化的新方法，并且还提供了一种通过使用上述方法来筛选各种化学品和天然材料的方法，以便选择针对TRAP磷酸化的抑制剂。预期该抑制剂将用于金黄色葡萄球菌的新型抗生素，因为这种TRAP磷酸化对于感染金黄色葡萄球菌是必不可少的。因此，在不久的将来，本发明可广泛用于开发抗金黄色葡萄球菌及其抗性菌株的新药。

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公开/公告号US2009075308A1

专利类型
公开/公告日2009-03-19

原文格式PDF
申请/专利权人 KYEONG KYU KIM;SUNG WOOK KANG;
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申请/专利号US20080185843
发明设计人 SUNG WOOK KANG;KYEONG KYU KIM;
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申请日2008-08-05
分类号C12Q1/48;C12P21/00;C12Q1/66;
国家 US
入库时间 2022-08-21 19:34:55

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