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METHOD FOR NONINVASNELY AND QUANTITATIVELY MONITORING THERAPEUTIC AND DIAGNOSTIC TRANSGENE EXPRESSION INDUCED BY EX VNO AND IN VNO GENE TARGETING IN ORGANS, TISSUES AND CELLS
METHOD FOR NONINVASNELY AND QUANTITATIVELY MONITORING THERAPEUTIC AND DIAGNOSTIC TRANSGENE EXPRESSION INDUCED BY EX VNO AND IN VNO GENE TARGETING IN ORGANS, TISSUES AND CELLS
An composition in a method for noninvasively monitoring the expression of therapeutic transgene delivered ex vivo and in vivo for the treatment of diseases includes the step of quantitatively imaging a reporter gene expression which is coupled to a therapeutic gene on a plasmid vector to infer levels, location, or duration of the therapeutic gene expression in the targeted tissues or organs. The reporter gene is imaged using a radiopharmaceutical for scintigraphic imaging of the gene expression interactions with the reporter gene, namely positron emission tomography, gamma camera or single-photon emission computed tomography. The genes are delivered with a liposome encapsulated reporter-therapeutic linked transgene vector with balanced reporter/therapeutic transgene expression. A transgene composition includes the reporter gene linked to the therapeutic gene or genes incorporated in and delivered by a liposome encapsulated reporter-therapeutic linked transgene vector.
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