The calibration tool is provided for use with a detector, such as a detector for detecting trace amounts of one or more substances of interest. The calibration tool includes a body with at least one reservoir for retaining the calibration solution therein. A nib projects from the body and communicates with the reservoir. The nib can be wiped across a detection surface and the detection surface then may be presented to a detector. The detector then can be calibrated for the particular substance of interest in the calibration solution. The calibration tool may include plural reservoirs isolated from one another and plural nibs for applying the calibration solution to a detection surface.
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