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Probe for detecting and quantifying lipid second messenger and method of detecting and quantifying lipid second messenger using the same
Probe for detecting and quantifying lipid second messenger and method of detecting and quantifying lipid second messenger using the same
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机译:用于检测和定量脂质第二信使的探针以及使用该探针检测和定量脂质第二信使的方法
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摘要
The present invention provides probes for detection and quantification of a lipid second messenger, which comprises: a polypeptide specifically bound to the lipid second messenger; two chromophores respectively having different fluorescence wavelengths, wherein each of the chromophores is linked to each end of the polypeptide through a rigid linker sequence; and a membrane localization sequence linked to one of the chromophores through a rigid linker sequence. According to the present invention, it is now possible to quantitatively detect when and in which site of a living cell the lipid second messengers are produced.
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