Process for producing functional non-naturally occurring proteins, and method for site-specific modification and immobilization of the proteins

摘要

There is provided a process for industrial production of non-naturally occurring proteins composed of less than 20 amino acids, wherein the proteins retain their original functions while being capable of site-specific modification or immobilization, or having new functions not found in nature in addition to the original functions of the proteins.;Specifically there are provided a process for producing a functional non-naturally occurring protein having a specific amino acid type(s) replaced with a natural amino acid(s) other than the amino acid type(s), the process comprising:a) matching a nucleic acid portion having a nucleotide sequence reflecting the genotype with a protein portion that is the translation product of the nucleic acid portion;b) selecting the matched molecule obtained in step a);c) introducing mutation into the nucleic acid portion of the matched molecule obtained in step b);d) amplifying the nucleic acid portion obtained in step c);e) providing the nucleic acid portion obtained in step d) to step a), to match the nucleic acid portion with a protein portion that is the translation product of the nucleic acid portion; andf) selecting the matched molecule obtained in step e), to produce a functional non-naturally occurring protein;and a method for site-specific modification and site-immobilization of a functional non-naturally occurring protein.