As160 type Protein, Test Systems, and their use in Methods for the identification of Therapies for Type 2 diabetes

摘要

Similar to the substrate Protein of 160 kDa protein kinase AKT (Protein as160 type)A method for identifying a substance that Alters the uptake of glucose and / or translocation of GLUT4 to the plasma membrane of a cell that comprises contacting a Test System, which includes the like Protein of 160 kDa substrate of AKT (Protein such as 160) with u The test substance,And identify the test substance as a substance that Alters glucose uptake of a cell through the detection of a signal that indicates an alteration of glucose uptake of the cell; a test system comprising a Gene that encodes a Protein similar to the deducted AKT to 160 (160 kDa Protein as type) and an inducible Promoter that provides the expressionTrolable Gene; use of the test system for the identification of a substance that enhances glucose uptake and / or translocation of GLUT4 to the plasma membrane of a cell; and use of the 160 type Protein as a model for Type 2 diabetes.