The process to differentiate conventional soybean soybean resistant to glyphosate

摘要

The process to differentiate conventional soybean soybean resistant to glyphosate.The present invention provides a process, sensitive and reliable, in which applies high performance liquid chromatography to detect acid chiquimico tissues (leaves, roots or rootlets) of soy with specific use to discriminate varieties of soybeans that geneti conventional Cally modified, resistant to glyphosate.Different experimental protocols can be applied to the analysis of chiquimico acid into the chromatograph, and the use of each depends on the chosen plant tissue.The invention has been developed based on the fact of shikimic acid accumulate significantly in the conventional soybean plant tissues after treatment with glyphosate herbicide that inhibits a key enzyme in the metabolism of shikimic acid.In turn, the genetically modified soybean resistant to glyphosate, and insensitive to this herbicide and, therefore, does not accumulate shikimic acid in any of the experimental conditions described in the invention.By high performance liquid chromatography, the shikimic acid can be detected in quantities of extremely low (in the range of 109 G), allowing to discriminate efficiently conventional soybeans genetically modified soybean resistant to glyphosate.