Sepn. of von Willebrand factor (vWF) into high and low mol. wt. fractions comprises binding vWF to an affinity column and carrying out elution at different salt concns. Also new are: (1) low mol. wt. fraction (A) of vWF, consisting mainly of dimers and tetramers and (2) high mol. wt. fraction (B) of at 50 (pref. 60)% increased activity (per mu g. of protein) in platelet aggregation compared with the physiological mixt. of both forms.
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