A PROCESS FOR THE PREPARATION OF NOVEL ANTIFUNGAL METABOLITES FROM NOVEL STRAIN OF PSEUDOMONAS ELUOROSCENCE USEUL FOR CONTROLLING FUNGAL DISEASE

摘要

A process for the preparation of noval antifungal metabolites •from novel strain of pseudomonas fluorescence useful for controlling fungal. diseases which comprises (a) isolating novel rhizosphere-competent blocontrol strain of Pseudomonas fluorescens having characteristics as herein descri-bed by conventional methods from the roots of leguminous plants growing the said isolated strain in a conventional nutrient medium at a temperature in the range of 20 C to 40 C for a period of 12 hrs to 168 hrs, under stirring at revolution per minute (RPM) upto 280 RPM. centrifuging at a speed in the range of 2000 to 20000 RPM for aeriod in the range of 10 to 60 minute to separate the superatant from residue and extracting the anti-fungal metabolite using organic solvent such as herein described and recovering the antifungal metabolite by evaporating the solvent.