RAPID VISUAL ASSAY FOR DISTINCT IDENTIFICATION OF HIV-1 amp; 2 ANTIBODIES AND HIV P24 ANTIGEN

摘要

a. applying antibody to HIV-p24 antigens in one dot and different antigens as herein described of HIV-1 & HIV-2 as separate or single dot at respective position and applying anti-human IgG as one dot for control dot on nitrocellulose membrane of assay device, b. applying patient serum/plasma on to said membrane, c. applying wash buffer as herein described to remove no-specific or unwanted antibody binding to the said membrane, d. applying conjugated colloidal gold on the said membrane, e. applying wash buffer again to said membrane to develop colour dot on the said membrane and confirm the presence of HIV-p24 antigen and antibodies to HIV-1 and HIV-2 antigens.