ISOLATION OF SAPONIN NEW COMPOUND FROM ACACIA CONCINNA DC

摘要

The invention relates to the antifertility activity of acetone fraction of aqueous extract of Acacia concinna. The LD50 and ED50 were observed 500 mg/kg and 50 mg/kg respectively. Female albino rats were orally fed with fraction (50 mg/kg) for 21 days throughout the study. Rats were arrested in diestrus stage and observed decrease in body weight and organs weight. Reduction in pregnancy and in implantation were observed in rats. Abortifacient activity was not observed with the treatment of fraction. Biochemical, hormonal, proteins present and pH in intrauterine fluid and histological studies were carried out. The levels of serum cholesterol and triglycerides decrease significantly. Rats showed depletion in follicle stimulating hormone, leutinizing hormone and estrogen levels. Antithyroid study showed the fraction was not similar to goiterogenic compounds. Proteins, which required for implantation were found to be absent. Decrease in the pH value of intrauterine fluid, explain the inactivity of sperms. Histological architecture of the uterus showed regressed endometrial glands. Ovary showed degeneration of developing follicle. Epithelial cells of the fallopian tube showed degeneration and accumulation in the lumen. Vagina showed marked changes in the epithelial layers. It can be concluded that the acetone fraction of aqueous extract of Acacia concinna is used as herbal contraceptive without any undesirable side effect.