A PROCESS FOR THE PREPARATION OF ORAL INSULIN MICROCAPSULES

摘要

WE CLAIM: 1. A process for the preparation of oral insulin microcapsules comprising emulsifying 1 to 10% solution of chitosan in a vegetable oil; treating with a solidifying agent such as a solution of tripolyphosphate or sodium hydroxide to obtain the chitosan microparticles; filtering and washing the chitosan microparticles; encapsulating the microparticles in alginate solution comprising mixing 100-750 mg chitosan microparticles and 20% slurry of calcium citrate with a 2-6% solution of aliginic acid to obtain a mixture, emulsifying the mixture in a vegetable oil to form capsules, partioning the capsules in a solution of calcium chloride, filtering, washing and drying to obtain the encapsulated microspheres. loading the encapsulated microspheres with 40 to 2000 IU/ml of insulin for a period of 4-16 hrs. at a temperature in the range of 2-6°C, by diffusion filling method to obtain the oral insulin microcapsules.