AN IMPROVED PROCESS FOR THE SINGLE STEP ISOLATION OF ALKALINE PROTEASE FROM A FERMENTATION BROTH

摘要

A process for the single step isolation of alkaline protease was developed us:::2 commercially available hydrophobic ligands. Higher adsorption was obtained oa butyl and phenyl hydrophobic ligands (94 and 98% respectively). Almost 20 fold purification with 40% yield of alkaline protease was obtained using gradient eiulion in a single slep operation.