Heptaazotate of hexa-(µ-?-alanine -(O,O'))-µ3-oxo-tri(aqua)triferrum(III) 3,5-hydrate and process for Spirulina platensis biomass obtaining

摘要

The invention relates to a new chemical compound as ferrum content regulator in the cultivation of Spirulina platensis biomass used in medicine and food industry.A chemical compound is claimed, namely heptaazotate of hexa-(µ-β-alanine-(O,O'))- µ3-oxo-tri(aqua)triferrum(III) 3,5-hydrate as ferrum content regulator in the cultivation of Spirulina platensis cyanobacterium biomass.A process for Spirulina platensis biomass obtaining is also claimed, including preparation of the nutrient medium containing, g/l: NaHCO3 - 16,8; K2HPO4·3H2O - 1,0; NaNO3 - 2,5; NaCl - 1,0; K2SO4 - 1,0; CaCl2·6H2O - 0,04; MgSO4·7H2O - 0,20; H3BO3 - 0,00286; MnCl2·4H2O - 0,00181; ZnSO4·7H2O - 0,00022; CuSO4·5H2O - 0,00008; MoO3 - 0,000015 and water - the rest. For inoculation is used 0,4 g/l suspension of Spirulina platensis and it is cultivated during 6 days at the temperature of 30…35ºC, with an illumination of 3000…4800 lx and medium pH 9,5…10,0. Into the nutrient medium there is additionally added heptaazotate of hexa-(µ-β-alanine-(O,O'))- µ3-oxo-tri(aqua)-triferrum(III) 3,5-hydrate 0,04…0,05 g/l, batchwisely, in the first four days of cultivation.