Integrase fusion proteins and their use with integrating gene therapy

摘要

Disclosed is an in vitro method of targeting integration of a transgene comprising retrovirus-like nucleic acid into a eukaryotic genome, in which the genome is targeted by a restriction enzyme that bind nucleic acid and the transgene is introduced at the binding site, wherein the endonuclease is specific to a site in an abundant rDNA locus and is fused to an integrase that mediates the introduction of the transgene.