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CHIMERIC HUMAN PAPILLOMAVIRUS 16 L1 PROTEINS COMPRISING AN L2 PEPTIDE, VIRUS-LIKE PARTICLES PREPARED THEREFROM AND A METHOD FOR PREPARING THE PARTICLES
CHIMERIC HUMAN PAPILLOMAVIRUS 16 L1 PROTEINS COMPRISING AN L2 PEPTIDE, VIRUS-LIKE PARTICLES PREPARED THEREFROM AND A METHOD FOR PREPARING THE PARTICLES
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机译:包含L2肽的嵌合人类乳头瘤病毒16 L1蛋白,制备病毒样颗粒的方法及其制备方法
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摘要
A method for producing a chimaeric human papillomavirus (HPV) L1 polypeptide containing a heterologous peptide, and in particular, a HPV L2 peptide comprising the steps of introducing a DNA sequence coding for the heterologous peptide into a DNA sequence coding for the L1 polypeptide; introducing the DNA sequence including the sequences for the L1 polypeptide and heterologous peptide into a host cell in which the DNA sequence can be expressed; causing expression of the DNA sequence; and recovering the resulting chimaeric L1 polypeptide which includes the heterologous peptide. The invention also describes a vector for use in the method, a host cell containing the vector, and a vaccine including the chimaeric HPV L1 polypeptide produced according to the method.
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