Reducing interference uin by migration shift assay.

摘要

Method for detecting or identifying an analyte of interest in a sample, the method comprising: (i) contacting the sample containing the analyte with one or more conjugates of an affinity molecule and a charged carrier molecule, wherein the molecule affinity is capable of binding the analyte to thereby form a complex of the analyte and one or more conjugates; (Ii) separating the complex from any unbound conjugate in the presence of a charged polymer using a separation channel in a microfluidic device comprising at least one separation channel having at least one microscale dimension of between about 0.1 and 500 .mu.m; and (iii) detecting the complex to identify the presence of the analyte or to determine the amount of analyte in the sample, wherein the charged carrier molecule is capable of causing a change in the migration time of the analyte by binding to form a complex of the analyte , the affinity molecule and the charged carrier molecule; and wherein the charged polymer has the same charge as the charged carrier molecule, so that the charged polymer interferes with the binding of sample components having an opposite that of the charged carrier molecule charge.