Procedure for cell detection high performance interactions between nuclear receptors and ligands.

摘要

Fusion protein comprises at least three domains, in which - a first domain mediates the localization of the fusion protein in the membrane in a cellular context and has or is derived from an amino acid sequence of a signal of membrane localization or a transmembrane domain, - a second domain has, or presumably has, a function of ligand binding of a nuclear receptor and comprises or is derived from an amino acid sequence segment receiver of a steroid receptor, an orphan receptor, a receptor vitamins, receptor thyroxine a dioxin receptor or retinoic acid receptor, - a third domain has an activity capable of activating a subsequent signaling pathway to a Ras protein in a cell and comprises an amino acid sequence derived from the amino acid sequence of this Ras protein in nature or a nucleotide exchange factor guanine present in the nat uraleza, characterized in that in the absence of ligand binding to the second domain of the fusion protein, the third domain can not develop, despite its membrane localization, activity of activating a subsequent signaling pathway to a Ras protein in cell, but in the case of ligand binding to the second domain, a conformational change effects on the third domain so it occurs that the third domain can develop their activation activity subsequent signaling pathway to a Ras protein in the cell .