首页> 外国专利> PROCEDURE AND NUCLEIC ACIDS FOR THE ANALYSIS OF A PROLIFERATIVE DISORDER OF COLON CELLS.

PROCEDURE AND NUCLEIC ACIDS FOR THE ANALYSIS OF A PROLIFERATIVE DISORDER OF COLON CELLS.

机译:用于分析结肠细胞增殖性疾病的程序和核酸。

摘要

A method for detecting and differentiating between proliferative disorders of colon cells comprising the following steps: a) providing a sample of body fluid containing genomic DNA, b) extracting said genomic DNA, c) converting the cytosine bases of said genomic DNA sample that are demethylated in position 5, by treatment, in uracil or in another base that is different from cytosine in terms of the behavior in base pairing and d) identify the methylation status of one or more cytosine positions of the TPEF gene and / or its regulatory regions.
机译:一种用于检测和区分结肠细胞增殖性疾病的方法,包括以下步骤:a)提供含有基因组DNA的体液样品,b)提取所述基因组DNA,c)转化所述基因组DNA样品中脱甲基的胞嘧啶碱基在位置5中,通过治疗,在尿嘧啶或在碱基配对行为方面不同于胞嘧啶的另一碱基中,和d)鉴定TPEF基因和/或其调节区的一个或多个胞嘧啶位置的甲基化状态。

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