The invention relates to a system for expressing a transgene in a target cell or a human or animal tissue characterized in that it consists of a eukaryotic cell and established in line, in which were transfected: a) a recombinant viral sequence in which a gene has been deleted in whole or in part and substituted by said transgene at this gene; b) a nucleic acid sequence including a sequence encoding the deleted protein, which sequence is under the control of a promoter and is associated where appropriate to said transgene, and flanked at its 3 'end a polyadenylation site; said recombinant viral genome and said sequence, carried by one or two plasmid supports, being capable of complementing each other in trans and allowing said host cell to produce defective infectious viruses.
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