首页>
外国专利>
METHOD FOR SEPARATION AND IDENTIFICATION OF BIOMOLECULES USING UNCONVENTIONAL GEL ELECTROPHORESIS AND DETECTION OF SINGLE NANOPARTICLE PROBES
METHOD FOR SEPARATION AND IDENTIFICATION OF BIOMOLECULES USING UNCONVENTIONAL GEL ELECTROPHORESIS AND DETECTION OF SINGLE NANOPARTICLE PROBES
展开▼
机译:非常规凝胶电泳分离和鉴定单个纳米粒子的生物分子鉴定方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
A method for identifying and separating of target biomolecules is disclosed. The method involves contacting a sample with a nanoparticle probe that is capable of specifically binding a target biomolecule. The complex formed by the nanoparticle target biomolecule is separated by electrophoresis using a non- conventional gel composed of a synthetically cross-linked polymer and a gel stabilizer. The nanoparticle probe:target biomolecule complex is detected, thereby identifying the target biomolecule. In certain examples, the nanoparticle probe emits a signal to identify its location. Particular examples of nanoparticle probes are semiconductor nanocrystals, such as quantum dots, which fluoresce at a particular wavelength. Also disclosed is a method of substantially sealing and/or increasing the transparency of a membrane. Such a method includes applying a film to a membrane. Sealing the membrane allows the detection of single nanoparticles.
展开▼