A METHOD FOR DETERMINING IMPORTANCE OF FRACTIONS OF BIOLOGICAL MIXTURES SEPARATED BY A CHROMATOGRAPHIC METHOD FOR DISCRIMINATION OF CELL OR TISSUE PHYSIOLOGICAL CONDITIONS

摘要

The invention describes a method for determining importance of fractions of biological mixtures separated by a chromatographic method for discrimination of cell cultures or tissues differing in physiological conditions. The method relies on mu ltiple measurements for each condition and comprises a sequence of computational steps performed on data sets derived from chromatograms. The data set is processed in the following sequence: (i) applying a projection technique to the data set, (ii) evaluating importance of components in the projected space using unsupervised and/or supervised approaches, (iii) back-projecting only a selected subset of the components, thereby reducing influence of noise and systemic biases in the data set, and (iv) determining importance of fractions using a feature selection method. In addition to accurate recognition of important regions of chromatogram, the invention provides efficient tissue discrimination models, improved visualization, and has applications in medical diagnostics, quality control and basic biomedical science.